Essential collection

Specimen collection: an essential tool.

Heterozygous Yeast Deletion Collection Screens Reveal Essential Targets of Hsp90

Hsp90 is an essential eukaryotic chaperone with a role in folding specific "client" proteins such as kinases and hormone receptors. Previously performed homozygous diploid yeast deletion collection screens uncovered broad requirements for Hsp90 in cellular transport and cell cycle progression. These screens also revealed that the requisite cellular functions of Hsp90 change with growth temperat...

The effect of collection time on essential oil of Oliveria decumbens Vent.

Background and objectives: Oliveria decumbens belongs to Apiaceae family and is used in wide range of diseases such as gastrointestinal disorder. Several studies have shown that Oliveria decumbens essential oil possessed high amount of thymol and carvacrol but some other studies revealed that myristicin was one of the main components of the oil. The aim of the...

Impossibility of ‘Essential’ Real-Time Garbage Collection in the General Case

We give an example of a computational problem for which no RAM (random access machine) can ensure a non-trivial garbage collection though a large amount of used memory can be in principle freed starting from some time moment. This example is of theoretical nature and means only that there can be no universal algorithm for ‘sufficiently good’ realtime garbage collection applicable in all situati...

An Updated Collection of Sequence Barcoded Temperature-Sensitive Alleles of Yeast Essential Genes.

Systematic analyses of essential gene function using mutant collections in Saccharomyces cerevisiae have been conducted using collections of heterozygous diploids, promoter shut-off alleles, through alleles with destabilized mRNA, destabilized protein, or bearing mutations that lead to a temperature-sensitive (ts) phenotype. We previously described a method for construction of barcoded ts allel...